Temporal Regulation of Gene Expression in the Mammary Gland and other Secretory Tissues (MMTV-LTR/tTA transgene)
Summary
Background
Key Citations
Hennighausen, L., Wall, R.J., Tillamnn, U., Li, M. and Furth, P.A. (1995). Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system. J. Cell. Biochem. 59:463-472.
Transgene
mouse strain
The transgenes were introduced into a Bl6xSJLbackground.
Mammary Development
Females were able to nurse and raise their litters. Whole mount and histology did not indicate any aberrant mammary development.
Tissue distribution of transgene (lacZ) expression
Beta-galactosidase activity was analyzed in tissues from mice that were transgenic for MMTV-LTR-tTA and tetop-nlacZ. Highly heterogenous expression was observed in mammary tissue.
Expression of the tetop-lacZ transgene was more homogenous in the salivary gland and in seminal vesicle.
key words
tet system, MMTV-LTR, lacZ, mammary gland, inducible gene expression
Submitted
by Lothar Hennighausen(e-mail mammary@nih.gov)
last update: January 7, 1996
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