Temporal Control of Gene Expression in Transgenic Animals using Tetracycline Responsive Systems

This page provides information on the use of tetracycline responsive systems to control temporal gene expression in transgenic mice. For additional information on vectors and cell lines see the Bujard page.

Review Articles

overview article by Lothar Hennighausen and Priscilla Furth

Diverse strategies for tetracycline-regulated inducible gene expression by Penny Shockett and David Schatz

The single step integration tet responsive system by the Bluethmann lab

Temporal Regulation of Gene Expression with the RU486 system by Steven S. Chua et al.

Transgenic research data

Time sensitive activation of the SV40 T antigen in the salivary gland

regulating temporal expression with the tet responsive system in the mammary gland and other secretory tissues

the autoregulatory tetracycline system

Transgenic mice

tTA under contol of the HCMV-IE1 enhancer (Max-Planck, NIH)
tTA under control of the MMTV-LTR (NIH, USDA) tTA under control of the insulin gene promoter (Einstein)
tTA expression in the CaM kinase promoter (Columbia)
tTA under autoregulatory tetracycline system control (Yale)
lacZ expression under tetop control (Max Planck, NIH)
SV40 T antigen expression under tetop control

Research groups working with tet systems

Where to obtain mice carrying carrying the tet system

1. MMTV-tTA mice
These mice carry a hybrid gene containing an MMTV-LTR and the tTA gene (reference).

1. tetop-lacZ mice
These mice carry a hybrid gene containing the tetop promoter and the coding region for beta-galactosidase with a nuclear translocation signal (reference).

1. tetop-luci mice
These mice carry a hybrid gene containing the tetop promoter and the coding region for luciferase (reference).

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Note: We have provided these mice also to the following investigators who should willing to pass them on.

Mark Bix (e-mail mbix@socrates.ucsf.edu)
tel. 415-476-6309

David Bodine (e-mail tedyaz@nchgr.nih.gov)
tel. 301-402-0902

Bruce Conklin (e-mail bruce_conklin.gicd@quickmail.ucsf.edu)

Mitchel Klebig (e-mail mklebig@wizard.hosp.uth.edu)
tel. 615-544-9165

John Roder (e-mail roder@mshri.on.ca)

Myung Shin (Tilghman lab) (e-mail mshin@mousegenome.princeton.edu)
tel. 609-258-2899

Suzanne Tharin (e-mail tharin@cshl.org)
tel. 515-367-8168

Miles Wilkinson (e-mail miles_wilkinson@isqm.mda.uth.tmc.edu)

Dong-Er Zhang (e-mail dzhang@bih.harvard.edu)

where to obtain DNA plasmids carrying the tet system
The original DNA constructs generated in the Bujard lab should be requested from Professor Hermann Bujard (FAX: 49-6221-565892) who's lab recently placed a trouble shooting guide on the Web.

Tetracycline pellets can be obtained from Innovative Research of America (e-mail pellets@innovrsrch.com)
their Web site