Hennighausen, L. (1992). The potential to domesticate milk protein genes. J. Cell. Biochem. 49, 325-332.

McKnight, R.A., Burdon, T., Pursel, V.G., Shamay, A., Wall, R.J. and Hennighausen, L. (1991). The whey acidic protein. In R.B Dickson and M.E. Lippman (eds.) Breast Cancer: Molecular and Cellular Biology, Kluwer Academic Publishers, Boston, 399-412.

Li, M., Hu, J., Heermeier, K., Hennighausen, L. and Furth, P.A. (1996). Apoptosis and remodelling of mammary gland tissue during involution proceeds through p53 independent pathways. Cell Growth & Differ., in press.

Robinson, G.W., McKnight, R.A., Smith, G.H., and Hennighausen, L. (1995) Mammary epithelial cells undergo secretory differentiation in cycling virgins but require pregnancy for the establishment of terminal differentiation. Development 121: 2079-2090.

Paleyanda, R.K., Zhang, D.-W., Hennighausen, L., McKnight, R. and Lubon, H. (1994) Regulation of human protein C gene expression by the mouse WAP gene promoter. Transgenic Research 3, 335-343.

Bischoff, R., Degryse, E. et al. (1992). A 17.6 kb region located upstream of the rabbit WAP gene directs high level expression of a functional human protein variant in transgenic mouce milk. FEBS Letters 305, 265-268.

Tzeng, Y. -J., E. Guhl, M. Graessmann, and A. Graessmann (1993). Breast cancer formation in transgenic animals induced by the whey acidic protein SV40 T antigen (WAP-SV-T) hybrid gene. Oncogene 8:1965-1971.

Velander WH, Page RL, Morcol T, Russell CG, Canseco R, Young JM, Drohan WN, Gwazdauskas FC, Wilkins TD and Johnson JL. Production of biologically active proteinC in the milk of transgenic mice. Ann N Y Acad Sci 665: 391-403, 1992.
Pittius, C.W., Sankaran, L., Topper, Y.J. and Hennighausen. (1988). Comparison of the regulation of the whey acidic protein gene to a hybrid gene containing the whey acidic protein gene promoter in transgenic mice. Mol. Endocrinology 2, 1027-1032.

Pittius, C., Hennighausen, L., Lee, E., Westphal, H., Nicols, E., Vitale, J. and Gordon, K. (1988). A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice. Proc. Natl. Acad. Sci. U.S.A. 85, 5874-5878.

Gordon, K., Lee, E., Vitale, J.A., Smith, A.E., Westphal, H. and Hennighausen, L. (1987). Production of human tissue plasminogen activator in transgenic mouse milk. BIO/TECHNOLOGY 5, 1183-1187.

Andres, A.-C., SchÖnenberger, C.A., Groner, B., Hennighausen, L., LeMeur, M. and Gerlinger, P. (1987). Ha-Ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation and tumor induction in transgenic mice. Proc. Natl. Acad. Sci. U.S.A. 84, 1299-1303.

Introduction of a target gene into the 1st exon of the WAP gene

Sandgren, E.P., Schroeder, J.A, Qui, T.H., Palmiter, R.D., Brinster, R.L. and Lee, D.C. (1995). Inhibition of mammary gland involution is associated with transforming growth factor a but not c-myc-induced tumorigenesis in transgenic mice. Cancer Res. 55, 3915-3927.

Kordon, E.C., McKnight, R.A., Jhappan, C., Hennighausen, L., Merlino, G. and Smith, G.H. (1995). Ectopic TGF-b1 expression in the secretory mammary epithelium induces early senescence of the epithelial stem cell population. Dev. Biol. 168, 47-61.

Jhappan, C., Geiser, A.G., Kordon, E.C., Bagheri, D., Hennighausen, L., Roberts, A.B., Smith, G.H. and Merlino, G. (1993). Targeting expression of a transforming growth factor b1 transgene to the pregnent mammary gland inhibits alveolar development and lactation. EMBO J. 12, 1835-1845.

Substituting WAP gene exons for target genes

Li, B., Greenberg, N., Stephens, L.C., Meyn, R., Medina, D. and Rosen, J.M. (1994). Preferential overexpression of a 172Arg-Leu mutant p53 in the mammary gland of transgenic mice results in altered lobuloalveolar development. Cell Growth & Differ. 5, 711-721.

Sympson, C.J., Talhouk, R.S., Alexander, C.M., Chin, J.R., Clift, S.M., Bissell, M.J. and Werb, Z. (1994). Targeted expression of stromelysin-1 in mammary gland provides evidence for a role of proteinases in branching morphogenesis and the requirement for an intact basement membrane for tissue-specific gene expression. J. Cell Biol. 125, 681-693.

Lothar Hennighausen (e-mail mammary@nih.gov)

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