Transgenes under control of the WAP gene promoter
Purpose
The genes encoding the whey acidic protein (WAP) from mouse (Campbell et al., 1984), rat (Campbell et al., 1984) and rabbit (Thepot et al., 1990) have been isolated and its promoters have been used to target the expression of transgenes to mammary tissue of transgenic animals (selected articles and reviews). Sequences can be extracted from Genbank. This page will describe and analyze different transgenes which have been investigated. Unpublished data will be marked as such. E-mail links to individual investigators have been generated. Information about WAP based expression vectors and hybridiztion probes can be found under WAP tools.
Three conceptually different types of hybrid genes have been tested in transgenic animals.
Fusion of a WAP gene promoter to the body of a target gene
The mouse (mWAP), rat (rWAP) and rabbit (raWAP) gene promoters have been used successfully to target the expression of hybrid genes to mammary tissue of transgenic animals (references). Expression of the following transgenes (in historical order) in mice has been published.
- mWAP-human ras [Andres et al., 1987]
- mWAP-human t-PA [Gordon et al., 1987]
- mWAP-human myc [Schoenenberger, 1987]
- mWAP-human growth hormone
- mWAP-human u-PA
- raWAP-human a1AT [Bischoff et al., 1992]
- mWAP-SV40 Tantigen [Li et al., 1996]
- mWAP-human proteinC [Paleyanda et al., 1994]
- raWAP-human a1AT [Devinoy et al., 1994]
- mWAP-lacZ [Robinson et al., 1995]
- raWAP-CD6-antibody [Limonta et al., 1995]
- mWAP-fucosyltransferase [Prieto et al., 1995]
- mWAP-int3 [Robinson et al., 1996]
- mWAP-int3 [Gallahan et al., 1996]
- rWAP-human surfactant [Yarus et al., 1996]
- mWAP-bcl2 [Jaeger and Weiher, unpublished]
- mWAP-human glucocerebrosidase [unpublished]
WAP gene promoter sequences
Transgenes 1-4 and 8 contain a 2.4 kb EcoRI - Asp718 mouse WAP gene promoter fragment. Asp718 cuts immediately 5' of the ATG within the first exon of the WAP gene.
Introduction of a target gene into the 1st exon of the WAP gene
The following cDNAs and genomic sequences have been inserted into the Asp718 (KpnI) site of the mouse (mWAP) gene (references).
- mWAP-human proteinC (cDNA) [Velander et al., 1992]
- mWAP-human TGFb1 (cDNA) [Jhappan et al., 1993]
- mWAP-human myc (genomic)
- mWAP-human TGFa (cDNA) [Sandgren et al., 1995]
- mWAP-human glucocerebrosidase [genomic, unpublished]
- mWAP-human u-PA [genomic, not published]
Introduction of a target gene between exon 1 and 3 of the WAP gene
The following cDNAs have been inserted between the Asp718 and SalI (exon 3) sites of the mouse (mWAP) gene (references).
- mWAP-ECSOD
- mWAP-stromelysin 1 [Sympson et al., 1994]
- mWAP-IGF1 [Neuenschwander et al., 1996]
- mWAP-stromelysin 3 [unpublished]
This information has been contributed by Lothar Hennighausen.
For additional information contact
Lothar Hennighausen (e-mail: mammary@nih.gov)
last update: March 11, 1996
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