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Tools and Techniques in Mammary Gland Biology


This page serves as source for tools, techniques and developments. If information from this page is used in publications, the URL of the mammary Web site should be cited.


  1. Animal Techniques
  2. Histological Techniques
  3. Molecular Techniques


1. Animal Techniques

1.1 Transplants of Adult Tissue into Fat Pads

1.2  Transplants of Embryonic Tissue into Fat Pads

1.3 Renal Capsule Grafting

1.4. Milking a Mouse

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2. Histological Techniques

2.1 Staining

2.2  Bromodeoxyuridine Staining of Mammary Tissue


3. Molecular Techniques

3.1 Markers to Probe Mammary Gland Differentiation

3.1.1 Molecular Markers

3.1.2 Protein Markers

3.2 RT-PCR Assays in Mammary Tissue

3.3 The tetracycline Responsive System

3.4  Deleting genes in mammary tissue with the Cre/lox system

3.4.1 Using the WAP Promoter

3.4.2 Using the BLG Promoter

3.5 Vectors for Targeting Transgenes to Mammary Tissue (WAP Gene Based)

3.6 Transgenes that have been Targeted to Mammary Tissue

3.7 Using WAP Gene Elements to study mammary gland development

3.8 Protocols

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