Summary
Insulin like growth factor-1 (IGF1) is a mitogen for several breast cancer cell lines, and
IGF1 receptors have been found on primary human breast cancer specimens. It has been
suggested that estrogen-independent breast cancer cell proliferation is mediated by
interactions between several growth factors, including IGF1, TGFA and TGFB. A family of
IGF binding proteins modulate IGF1 biological activity by sequestering or transporting the
growth factor. IGFBP3 is a major carrier of IGF1 and potentiates its actions.
Citation
unpublished
Background
Transgene
The targeting constuct consister of a rat prepro Igf1 cDNA ligated to a 7 kb EcoR1
fragment of the mouse Wap gene. The construct was microinjected into the pronuclei
of zygotes from (C57BL/6 x SJL)F1 females.
mouse strain
The transgene is expressed at high levels during lactation and post-lactation. Homozygotes are viable and fertile. There were no obvious changes in mammary gland histomorphology during lactation, but at 5 to 10 days post-laction, there was a delay in involution and decreased apoptosis.
Controls
The genetic background of this strain is mixed C57BL/6 x SJL. Normal wildtype siblings or
B6SJLF1/J mice may be used as controls. The B6SJLF1/J mice only provide an approximate
genetic match to this B6,SJL background.
Colony Maintenance
(This strain hasn't arrived in our IMR colony yet.)
Genetic Typing
For information on genotyping this strain, inquire by e-mail at micetech@jax.org or call
1-800-422-MICE.
key words
transgenic, WAP, whey acidic protein, mammary development, post-lactational
involution, Igf1, insulin-like growth factor 1
Originator
Dr. Derek LeRoith
National Institutes of Health
National Institute of Diabetes, Digestive, & Kidney Diseases
Building 10, Room 8S239
Bethesda, MD 20892-1770
Phone 301-496-8090
FAX 301-480-4386
Contact: derek@helix.nih.gov
Jackson lab ID
B6,SJL-TgN(WapIgf1)39Dlr (JR# 2500)
Information submitted by
Debbie Krupke: dmk@jax.org
Induced Mutant Resource,
The Jackson Laboratory.
on November 17, 1995